Kristen Li

The Amoeba Boys are a group of amoebas who, like their original counterparts, aren't very bright. The members are Bossman (the leader of the group), Slim (the skinniest amoeba), and Junior (the smallest amoeba). Their only major appearance was in "Viral Spiral" where they attempted to destroy the internet to make a name for themselves, but fail after Bubbles sends Blossom and Buttercup into the internet. They are all voiced by Chuck McCann reprising his roles from the original series with "Viral Spiral" marking his final role as them prior to his death on April 8, 2018.

Blossom is the leader of the Powerpuff Girls. Her appearance is similar to her original counterpart of the same name, with the exception of her ribbon, which is more rounded. She loves organization and hates when things are messy and out of order. She has a perfect attendance record and is an overachiever. Her unique abilities are ice breath, genius-level intelligence, microscopic vision, natural leadership skills, and intuitive aptitude. She also can project and manipulate bright pink energy, an ability her 1998 counterpart does not possess.

Bubbles is the sweetest of the Team. Bubbles has blonde hair and pigtails like her original counterpart, except with new blue hair ties and the pigtails being set slightly higher-up than previously. Bubbles is an animal lover like her 1998 counterpart and usually tries to save the day the nice way. Like her 1998 counterpart, she can be a bit naive but can also get angered very easily. When in bed she has Octi by her side. Her trail is blue with little circles. In "Viral Spiral", she is shown to have talent with computers and can even create and program her own video games. Her special abilities include telepathic communication with animals, zoolingulism, sound manipulation, sonic blasts, vocal mimicry, hypnotic singing, and multilingualism. She also can project and manipulate blue energy, an ability her 1998 counterpart does not possess.

Buttercup is the toughest yet goofiest of the Team. Her appearance is almost exactly like her original counterpart except she has a cowlick on the back of her head and a deeper voice. Buttercup is a tomboy who loves to get into action and likes to play sports, hanging out with boys and having fun like her 1998 counterpart, She has a fear of spiders like her 1998 counterpart as well. She has a temper that can usually get out of control. She is also the main archenemy of Manboy, who called her "princess", which she hates the most, Blossom and Bubbles attempt to stop her. Her trail is green with little triangles. Unlike her two sisters, she does not possesses any special abilities of her own. However, she can project and manipulate green energy, an ability her 1998 counterpart does not possess.

Professor Utonium is the overprotective father and creator of Bliss, Blossom, Bubbles, and Buttercup. He is a scientist who works in his lab at home. His appearance is similar to his 1998 counterpart, but his face now has round edges and he has two grey streaks in his hair. He loves the girls and supports them along the way.

The Rowdyruff Boys are the male counterparts of the Powerpuff Girls. They made a non-speaking cameo in the season 3 episode "Total Eclipse of the Kart". They are also identical to their 1998 counterparts' second appearances. The three Rowdyruff Boys are Brick, Boomer and Butch. Brick is the leader.